19F solid-state NMR approaches to probe antimicrobial peptide interactions with membranes in whole cells - ESPCI Paris - École supérieure de physique et de chimie industrielles de la ville de Paris Access content directly
Journal Articles Biochimica et Biophysica Acta:Biomembranes Year : 2024

19F solid-state NMR approaches to probe antimicrobial peptide interactions with membranes in whole cells

Kiran Kumar
Alexandre Arnold
Raphaël Gauthier
  • Function : Author
Marius Mamone
  • Function : Author
Jean-François Paquin

Abstract

To address the global problem of bacterial antibiotic resistance, antimicrobial peptides (AMPs) are considered promising therapeutic candidates due to their broad-spectrum and membrane-lytic activity. As preferential interactions with bacteria are crucial, it is equally important to investigate and understand their impact on eukaryotic cells. In this study, we employed 19 F solidstate nuclear magnetic resonance (ssNMR) as a novel approach to examine the interaction of AMPs with whole red blood cells (RBCs). We used RBC ghosts (devoid of hemoglobin) and developed a protocol to label their lipid membranes with palmitic acid (PA) monofluorinated at carbon positions 4, 8, or 14 on the acyl chain, allowing us to probe different locations in model and intact RBC ghost membranes. Our work revealed that changes in the 19 F chemical shift anisotropy, monitored through a C-F bond order parameter (S CF), can provide insights into lipid bilayer dynamics. This information was also obtained using magic-angle spinning (MAS) 19 F ssNMR spectra with and without 1 H decoupling, by studying alterations in the second spectral moment (M 2) as well as the 19 F isotropic chemical shift, linewidth, T 1 , and T 2 relaxation times. The appearance of an additional isotropic peak with a smaller CSA, a narrower linewidth, and a shorter T 1, induced by the AMP caerin 1.1, supports the presence of high-curvature regions in RBCs indicative of pore formation, analogous to its antimicrobial mechanism. In summary, the straightforward incorporation of monofluorinated FAs and rapid signal acquisition offer promising avenues for the study of whole cells using 19 F ssNMR.
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Dates and versions

hal-04386127 , version 1 (10-01-2024)

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Kiran Kumar, Alexandre Arnold, Raphaël Gauthier, Marius Mamone, Jean-François Paquin, et al.. 19F solid-state NMR approaches to probe antimicrobial peptide interactions with membranes in whole cells. Biochimica et Biophysica Acta:Biomembranes, 2024, 1866 (3), pp.184269. ⟨10.1016/j.bbamem.2023.184269⟩. ⟨hal-04386127⟩
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