Article Dans Une Revue PLoS ONE Année : 2014

ZFC3H1, a Zinc Finger Protein, Modulates IL-8 Transcription by Binding with Celastramycin A, a Potential Immune Suppressor

Résumé

Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins and investigated their biological functions. Our screening and subsequent pull-down assay revealed ZFC3H1 (also known as CCDC131 or CSRC2), an uncharacterized zinc finger protein, as a Celastramycin A binding protein. The knockdown of ZFC3H1 reduced IL-8 expression levels in the TNFα-stimulated lung carcinoma cell line, LU99, and UV-irradiated HeLa cells. Based on reporter assay results, we concluded that ZFC3H1 participates in the transcriptional activation of IL-8. The findings of our UV-irradiation experiments implied that ZFC3H1 may indirectly interact with ERCC1 in an activated DNA repair complex. Thus, we designated ZFC3H1 as a mammalian target of Celastramycin A (mTOC).
Fichier principal
Vignette du fichier
PDF Datastream-101.pdf (1.28 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
licence

Dates et versions

hal-03542270 , version 1 (04-02-2025)

Licence

Identifiants

Citer

Takeshi Tomita, Katsuaki Ieguchi, Frédéric Coin, Yasuhiro Kato, Haruhisa Kikuchi, et al.. ZFC3H1, a Zinc Finger Protein, Modulates IL-8 Transcription by Binding with Celastramycin A, a Potential Immune Suppressor. PLoS ONE, 2014, 9 (9), pp.e108957. ⟨10.1371/journal.pone.0108957⟩. ⟨hal-03542270⟩
13 Consultations
0 Téléchargements

Altmetric

Partager

More